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Flow cytometric analysis of CD8α expression on human peripheral blood lymphocytes. Human whole blood was stained with either BD Horizon™ BV786 Mouse Anti-Human CD8 antibody (Cat. No. 563823/563824; solid line histogram) or BD Horizon™ BV786 Mouse IgG1, κ Isotype Control (Cat. No. 563330; dashed line histogram). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.