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Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Human whole blood was stained with the BD Horizon™ BUV395 Mouse Anti-Human CD3 antibody (Cat. No. 563546/563548; solid line histogram) or with BD Horizon™ BUV395 Mouse IgG1, κ Isotype Control (Cat. No. 563547; dashed line histogram). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR™ II Flow Cytometry System.