Target Growth Hormone-Releasing Hormone Target background GHRH belongs to the glucagon family produced in the hypothalamus. It is essential for normal linear growth and has important metabolic effects throughout life. GHRH stimulates GH production and release by binding to the GHRH Receptor (GHRHR) on cells in the anterior pituitary. GHRH is released from neurosecretory nerve terminals of arcuate neurons, and is carried by the hypothalamo-hypophyseal portal system to the anterior pituitary gland, where it stimulates growth hormone(GH) secretion by stimulating the growth hormone-releasing hormone receptor. Target alias GHRH, Somatocrinin, Somatoliberin, GH-RH, Growth hormone–releasing factor (GHRF or GRF), Somatotropin-releasing hormone (SRH), Somatotropin-releasing factor (SRF), Somatorelin Immunogen synthetic rat GHRH (1-29)NH2 Specificity The antibody recognizes the rat GHRH and it shows no cross-reactivity with SRIF-14, SRIF-28 rGH, LH, FSH, PRL calcitonin, insulin, glucagon ovine CRF, secretin, vasoactive intestinal peptide, and cholecystokinin-8 Clone ID --- Preservative None Format Lyophilized serum Recommend starting dilution If reconstituted with deionized water in 50 µl: IHC: 1:15000; In vivo Passive Immunization: neat serum; RIA: In a RIA for rGRF-(1043), at a final dilution of 1: 785,000, this antiserum binds 35% of [125I]-rGRF and can detect 7pg/assay tube. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.