Target YY1 Target background YY1 is a ubiquitous and multifunctional zinc-finger transcription factor involved in the activation or repression of a large number of genes that are critical for basic biological processes of cell growth, development, differentiation, cell cycle and even programmed cell death (apoptosis). YY1 is an essential protein; deletion of YY1 leads to lethality in mouse embryos and disruption of one allele causing developmental defects. Immunogen Recombinant human YY1 Specificity The antibody recognizes the Transcription Factor YY1; in WB, the antibody recognizes the O-glycosylated, the phosphorylated, and the post-translationally unmodified protein in cellular and tissue lysates. Clone ID --- Preservative Ampicillin and streptomycin, 0.05% sodium aside. Format Total IgY purified, stored in PBS pH 7.4 and lyophilized. Recommend starting dilution If reconstituted with deionized water in 30 µl: WB 1:3000 – 1:8000; IF 1:500 - 1:1000 (in tissue paraffin sections). Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.