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Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human TGF-beta RII Fc Chimera (Catalog # 341-BR) is immobilized at 1 μg/mL (100 μL/well), it binds Recombinant Human (rh) TGF-beta RI/ALK-5 Fc Chimera in the presence of rhTGF-beta 1. The concentration of rhTGF-beta RI Fc Chimera that produces 50% of the optimal binding response is approximately 0.05-0.4 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human TGF-beta RI/ALK-5 protein
Human TGF-beta RI/ALK-5 (Ala25-Glu125) Accession # P36897 |
IEGRMD |
Human IgG1 (Pro100-Lys330) |
N-terminus | C-terminus | |
Accession #
N-terminal Sequence
Analysis
Analysis
Ala25 & Leu34
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
37 kDa (monomer)
SDS-PAGE
40-60 kDa, reducing conditions