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Ampligase® DNA Ligase Kit
  • 商城价格 登录后可查看价格
  • 货号 A8101
  • 品牌 LGC(Lucigen) ( 一级代理 )
  • CAS号
  • 规格/包装 1,000 U @ 5 U/ul
  • 单位
  • 储存条件 -20℃冷冻
  • 现货状态 一个月

  • 数量

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Ampligase™ Thermostable DNA Ligase catalyses NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures. Derived from a thermophilic bacterium, the enzyme is stable and active at much higher temperatures than conventional DNA ligases. Its half-life is 48 hours at 65°C and greater than 1 hour at 95°C. Ampligase DNA Ligase has been shown to be active for at least 500 thermal cycles (94°C/80°C) or 16 hours of cycling. 10 This exceptional thermostability permits extremely high hybridization stringency and ligation specificity. Ampligase DNA Ligase has no detectable activity in ligating blunt-ended DNA and has no activity on RNA or RNA:DNA hybrids. Applications Ligation amplification (Ligase Chain Reaction, LCR) Repeat Expansion Detection (RED) Simultaneous mutagenesis of multiple sites Other ligation-based detection methods
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