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Exonuclease VII has high enzymatic specificity for ssDNA and exhibits both 5´ to 3´ and 3´ to 5´ exonuclease activities. It is useful for rapid removal of single-stranded oligonucleotide primers from a completed PCR when different primers are required for subsequent PCR amplifications. Exonuclease VII digestion of ssDNA occurs in the absence of magnesium. Exonuclease VII can be inactivated by heating at 95 °C for 10 minutes.
