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Recombinant Mouse FGL2 HA-tag Protein,CF
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  • 货号 10691-FL-050
  • 品牌 R&D Systems ( 经销商 )
  • CAS号
  • 规格/包装 50ug
  • 单位 瓶
  • 储存条件
  • 现货状态 一个半月
  • 数量

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Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Fc gamma RIIB/CD32b  (Catalog # 1460-CD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse FGL2 HA-tag (Catalog # 10691-FL) binds with an ED50 of 0.1-0.8 µg/mL. Measured by its ability to inhibit anti-CD3-induced proliferation of mouse T lymphocytes. The ED50 for this effect is 1.5-12 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse FGL2 protein
Hemagglutinin Tag
(YPYDVPDYA) 		Mouse FGL2
(Val20-Pro432)
Accession # P12804.1
N-terminus C-terminus
Accession #
P12804.1
N-terminal Sequence
Analysis
Tyr of HA-tag and Asp 31
Predicted Molecular Mass
48 kDa
SDS-PAGE
61-72 kDa, under reducing conditions
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