Strep-Tactin®XT 4Flow® high capacity columns are applicable to the purification of Strep-tag®II or Twin-Strep-tag® fusion proteins via gravity flow. It is recommended for proteins of all classes, especially low abundant, large, and highly expressed proteins, due to its pH compatibility, target purity (> 95%), and high binding affinity. Multimeric protein complexes might bind too strongly to our Strep-Tactin®XT 4Flow® high capacity resins, preventing efficient elution. Strep-Tactin® resins are recommended for these proteins. With Strep-Tactin®XT 4Flow® high capacity, denaturing agents such as urea can be used. In comparison to Strep-Tactin®XT 4Flow®, Strep-Tactin®XT 4Flow® high capacity has a higher density of immobilized Strep-Tactin®XT, which leads to a higher yield of purified protein. Purify large samples faster with our WET FRED.