Strep-Tactin®XT 4Flow® high capacity is perfectly suited for purifying Strep-tag®II or Twin-Strep-tag® fusion proteins. This resin offers excellent pressure stability, pH compatibility, and target purity (> 95%). Additionally, this resin is compatible with a broad range of reagents. Its high binding affinity makes it ideal for low abundant, high-expressing and challenging proteins. A low concentration of agarose also facilitates the purification of large proteins (>90 kDa). When working with, for example, multimeric proteins, choosing a resin with the Strep-Tactin® ligand is recommended. Strep-Tactin®XT 4Flow® high capacity resin has a higher density of immobilized Strep-Tactin®XT than the classic Strep-Tactin®XT 4Flow®and thus can bind more protein. As the resin is optimized for column affinity chromatography, rather than batch purification, the suspension can be used to pack gravity flow columns.