Description:

An enzyme immunoassay for the quantitative measurement of pregnant mare serum gonadotropin (PMSG) in equine serum and plasma. The DRG PMSG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal antibody directed towards a unique antigenic site of the PMSG molecule.  An aliquot of sample containing endogenous PMSG is incubated in the coated well. After washing a second incubation follows with enzyme conjugate, which is an anti-PMSG antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off.  The amount of bound peroxidase is proportional to the concentration of PMSG in the sample. Having added the substrate solution, the intensity of color developed is proportional to the concentration of PMSG in the sample.

描述：
一种酶免疫测定法，用于定量测量马血清和血浆中怀孕母马血清促性腺激素 （PMSG）。DRG（靶点科技） PMSG ELISA 试剂盒是一种基于夹心原理的固相酶联免疫吸附测定 （ELISA）。微量滴定孔包被有针对 PMSG 分子独特抗原位点的单克隆抗体。 将含有内源性 PMSG 的样品等分试样在包被孔中孵育。洗涤后，进行第二次孵育，然后用酶偶联物孵育，这是一种与辣根过氧化物酶偶联的抗 PMSG 抗体。孵育后，将未结合的偶联物洗掉。 结合的过氧化物酶的量与样品中 PMSG 的浓度成正比。加入底物溶液后，显色的强度与样品中 PMSG 的浓度成正比。