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The cytochalasins are cell-permeable fungal metabolites that inhibit actin polymerization. This interferes with such diverse processes as cell movement, growth, phagocytosis, degranulation, and secretion. Cytochalasin J is a deacetylated analog of cytochalasin H that weakly inhibits actin assembly but significantly alters mitotic spindle microtubule organization and kinetochore structure. Like cytochalasin H, cytochalasin J suppresses the production of reactive oxygen species by stimulated human neutrophils.