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现货---Roche 480 SYBR快速定量试剂盒-1000 x 20 µL reactions (optimized for use with Roche LightCycler 480)
  • 商城价格 登录后可查看价格
  • 货号 A-KK4611
  • 品牌 kapabiosystems ( 经销商 )
  • CAS号 A-KK4611
  • 规格/包装 10ml
  • 单位 盒
  • 储存条件 低温
  • 现货状态 一周
  • 数量

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Kapa Biosystems was founded in 2006 to develop next-generation PCR reagents. Our molecular evolution technology platform is currently being used to optimize enzymes for DNA amplification, next-generation DNA sequencing, and molecular diagnostic applications.The majority of biomedical and research applications were designed around a small subset of native enzymes. The limitations of these applications are often the result of constraints due to enzyme structure or function. Recent advances in molecular evolution have allowed enzymes to be tailored to suit specific application requirements. Enzyme engineering improves existing methods and provides the foundation on which entirely novel applications can be built.Kapa Biosystems conducts advanced protein engineering, taking advantage of recent developments in genomics, proteomics, and synthetic biology.艾德科技联合KAPA Biosystems 能够为您提供性价比更好，更优的产品和服务.本产品相关信息详见如下：

KAPA SYBR® FAST qPCR Kits

Evolved to shine.

KAPA SYBR FAST qPCR Kits contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition.

The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis.  KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent real-time PCR reaction conditions, exhibit dramatic improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity.


Product Highlights

No bias in amplification efficiency across a wide range of GC contents (44.2 – 62.5%) was observed with KAPA SYBR FAST. Amplification efficiencies achieved for ten housekeeping genes with the KAPA SYBR FAST Universal qPCR Kit were plotted against GC content. The reaction efficiency achieved for each of the ten genes fell within the optimal range of 95% – 105%, independent of the nature of the amplicon. Data on file.
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Quantitate changes in gene expression more accurately

  • High reaction efficiency between 95 – 105% improves accuracy and reproducibility
  • Unbiased efficiency across a wide range of GC contents and amplicon lengths
Two target genes were amplified using a 10-fold dilution series of human genomic DNA (16 ng to 1.6 pg per 20 µL reaction) comparing four commercial kits. KAPA SYBR FAST consistently provides high performance for accurate expression quantitation across a range of difficult targets. Data on file.

Detect low copy and difficult targets consistently

  • Improved processivity results in earlier Cq scores
  • Higher fluorescence detection across varying AT- and GC-rich targets
  • Novel enzyme is resistant to SYBR inhibition
A 150 bp fragment of the human coagulation factor V gene was amplified from a set of five 10-fold dilutions of human genomic DNA (20 ng to 2 pg) with a standard-cycling protocol (green) and a fast-cycling protocol (blue). Data on file.

Complete real-time PCR runs in just 40 minutes

  • 55% shorter run times with fast cycling protocol
  • KK4611
    07959494001
    1000 x 20 µL reactions (optimized for use with Roche® LightCycler 480)
    10 mL

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