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Recombinant Human IL-6 Protein: 产品信息
纯度≥ 95 % as determined by SDS-PAGE
内毒素< 10 EU per mg of the protein.
生物活性1. Immobilized Human IL-6 (Cat:10395-HNAE) at 2 μg/mL (100 μL/well) can bind Human IL-6R hFc(Cat:10398-H02H), the EC50 of Human IL-6R hFc(Cat:10398-H02H) is 3.0-10.0 ng/mL.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.8 ng/mL.
3. Loaded Recombinant Human IL-6R Protein, His Tag (Cat. No. 10398-H08H) on HIS1K Biosensor, can bind Recombinant Human IL-6 Protein (Cat. No. 10395-HNAE) with an affinity constant of 75.9 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
蛋白构建A DNA sequence encoding the mature form of human IL6 (NP_000591.1) (Val30-Met212) was expressed with an initial Met at the N-terminus.
产品详情见链接：https://cn.sinobiological.com/recombinant-proteins/human-il-6-10395-hnae
纯度≥ 95 % as determined by SDS-PAGE
内毒素< 10 EU per mg of the protein.
生物活性1. Immobilized Human IL-6 (Cat:10395-HNAE) at 2 μg/mL (100 μL/well) can bind Human IL-6R hFc(Cat:10398-H02H), the EC50 of Human IL-6R hFc(Cat:10398-H02H) is 3.0-10.0 ng/mL.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.8 ng/mL.
3. Loaded Recombinant Human IL-6R Protein, His Tag (Cat. No. 10398-H08H) on HIS1K Biosensor, can bind Recombinant Human IL-6 Protein (Cat. No. 10395-HNAE) with an affinity constant of 75.9 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
蛋白构建A DNA sequence encoding the mature form of human IL6 (NP_000591.1) (Val30-Met212) was expressed with an initial Met at the N-terminus.
产品详情见链接：https://cn.sinobiological.com/recombinant-proteins/human-il-6-10395-hnae