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Lamin A/C Rabbit Polyclonal Antibody
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  • 货号 AWA42385-100ul
  • 品牌 abiowell ( 经销商 )
  • CAS号
  • 规格/包装 100ul
  • 单位 支
  • 储存条件
  • 现货状态 三个工作日
  • 数量

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产品简介：


pdf：https://www.abiowell.com/uploads/files/202402/65bf23725676b.pdf
html：

Product Details

 

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

74+65 kDa


Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml


Other Names:

LMNA; LMN1; Prelamin-A/C


Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


Purification:

Affinity-chromatography


Storage:

-20°C,1 year



Applications

 

WB 1:500-1:2000

IHC 1:100-1:300

IF 1:200-1:1000

ELISA 1:20000

Not yet tested in other applications.

 



Immunogen

Information

Gene Name：

LMNA

Protein Name：

Prelamin-A/C


Gene ID：

4000 (Human)     

16905 (Mouse)     

60374 (Rat)

SwissPro：

P02545 (Human)     

P48678 (Mouse)     

P48679 (Rat)

 


Subcellular Location：

Nucleus. Nucleus envelope. Nucleus lamina. Nucleus, nucleoplasm. Nucleus matrix.


Immunogen：

The antiserum was produced against synthesized peptide derived from human Lamin A/C. AA range:361-410.

 


Specificity:

Lamin A/C Polyclonal Antibody detects endogenous levels of Lamin A/C protein.




Product images
Lamin A/C Rabbit Polyclonal Antibody - 1 Fig : Immunohistochemical analysis of paraffin-embedded Rat-cerebellum tissue with Rabbit anti-Lamin A-C (AWA42385) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42385) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Lamin A/C Rabbit Polyclonal Antibody - 2 Fig : Immunohistochemical analysis of paraffin-embedded Rat-small intestine tissue with Rabbit anti-Lamin A-C (AWA42385) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42385) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Lamin A/C Rabbit Polyclonal Antibody - 3 Fig : Western blot analysis of Lamin A/C on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA42385, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell
Lane 2: COS7 cell
Lane 3: Hela cell
Lane 4: HUVEC cell
Lane 5: A375 cell
Lane 6: NIH3T3 cell


Exposure time: 7 seconds
Predicted band size: 65/74 kDa
Observed band size: 65/74 kDa

url：https://www.abiowell.com/duokelongkangti/AWA42385.html

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