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Dulbecco’s MEM (DMEM) Low Glucose, w/o Amino Acid
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  • 货号 D9800-13
  • 品牌 usb ( 经销商 )
  • CAS号 见包装
  • 规格/包装 10L
  • 单位 盒
  • 储存条件 见包装
  • 现货状态 三周
  • 数量

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Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Appearance
Light tan to orange, homogeneous, free-flowing powder
Solubility
Yellow to orange, clear, complete
pH
6.4 ± 0.2
Endotoxin
≤1EU/mg
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 10.04g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/Liter
Inorganic Salts
Calcium Chloride•2H2O 0.265
Ferric Nitrate•9H2O 0.0001
Magnesium Sulfate 0.09767
Potassium Chloride 0.4
Sodium Chloride 6.4
Sodium Phosphate Monobasic 0.109
Amino Acids
L-Arginine•HCl 0.084
L-Cystine•2HCl 0.0626
L-Glutamine 0.584
Glycine 0.03
L-Histidine•HCl•H2O 0.042
L-Isoleucine 0.105
L-Leucine 0.105
L-Lysine•HCl 0.146
L-Methionine 0.03
L-Phenylalanine 0.066
L-Serine 0.042
L-Threonine 0.095
L-Tryptophan 0.016
L-Tyrosine•2Na•2H2O 0.10379
L-Valine 0.094
Vitamins
Choline Chloride 0.004
Folic Acid 0.004
myo-Inositol 0.0072
Niacinamide 0.004
D-Pantothenic Acid•Ca 0.004
Pyridoxal•HCl 0.004
Riboflavin 0.0004
Thiamine•HCl 0.004
Other
D-Glucose 1
Pyruvic Acid, Sodium 0.11
Phenol Red, Sodium 0.0159
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