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| Cat. No. | T0132 |
| Name | Immortalized Human Lymphoblastoid Cells – EBV (KMS-9) |
| Description |
The Immortalized Human Lymphoblastoid Cells- EBV (KMS-9) was derived from peripheral human blood lymphocytes, with minimal genetic and phenotypic alterations compared to the parental lymphocytes. Being an actively proliferating B cell population, these cells provide a constant supply of starting materials suitable for various assays and in vitro model system for the study of human B cells. The KMS-9 cells express HLA-DR on its surface. It also exhibit slight expression of the CD19 B cell marker, but on the other hand, it is negative for CD20. Another lyphoblastoid cell line, KMS-15 (Cat. No. T0133), in contrast, expresses both CD19B and CD20 cell marker. |
| Organism | Human (H. sapiens) |
| Tissue | Blood |
| Donor History | Not disclosed |
| Growth Properties | Suspension, round clusters |
| Cell Type | Immortalized Cells |
| Storage Condition | Vapor phase of liquid nitrogen, or below -130°C. |
| Shipping Conditions | Ship with dry ice. |
| Product Format | Frozen |
| Intended Use | This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. |
| BioSafety | II |
| Certificate of Analysis | For batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com. |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow II (TM002) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. |
| Unpacking and Storage Instructions |
1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130°C, preferably in liquid nitrogen vapor phase storage, until ready for use. To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130°C or in liquid nitrogen vapor phase. Do not store at -70°C, as it will result in loss of viability. |
| Thawing Protocol |
1. Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask. 5. Incubate the cells at the recommended conditions. |
| Subculture Protocol |
1. Simply add fresh complete media directly to the culture. Do not allow cell density to exceed 1x10⁶ cells/ml. 2. Alternatively, replace complete growth media by centrifugation and re-suspend the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired. 3. Incubate the cells at the recommended conditions. |
| Cryopreservation |
Cryopreservation Medium (TM024), or complete growth media with 10% DMSO. |
| Immortalization Method |
Epstein-Barr Virus (EBV) |
| Expression |
HLA-DR, slight expression of CD19B |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T0132. |
| Warranty | abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”. |
| Disclaimer |
1. All test parameters provided in the CoA are conducted using abm's
standardized culture system and procedures. The stated values may vary
under the end-user's culture conditions. Please verify that the product
is suitable for your studies by referencing published papers or ordering
RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206,
$600.00) to perform preliminary experiments, or alternatively use our
Gene Expression Assay Service (Cat# C138). All sales are final. 2. We recommend live cell shipments for ease of cell transfer and this
option can be requested at the time of ordering. Please note that the
end-user will need to evaluate the feasibility of live cell shipment by
taking into account the final destination's temperature variation and
its geographical location. In addition, we thoroughly test our cell
lines for freeze-thaw recovery. If frozen cells were received and not
recovered in your lab under the exact, specified conditions (using
recommended culture vessel, media, additional supplements, and
atmospheric conditions), a live cell replacement is possible at a cost
(plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm
is not liable for any repercussions arising from the use of its cell
biology product(s) in therapeutic/diagnostic application(s). Please
contact a technical service representative for more information. 4. abm makes no warranties or representations as to
the accuracy of the information on this site. Citations from literature
and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 5. abm warrants that cell lines shall be viable upon
initiation of culture for a period of thirty (30) days after shipment
and that they shall meet the specifications on the applicable abm
Material Product Information sheet, certificate of analysis, and/or
catalog description. Such thirty (30) day period is referred to herein
as the "Warranty Period."
|
| Depositor | Kawasaki Medical School |
| Application | Research Use Only. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T0132 |