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Optimal Growth with Endothelial Cell Medium (ECM) and ECGS (sciencellonline.com)
Endothelial Cell Medium (ECM), when used with Endothelial Cell Growth Supplement (ECGS, Cat #1052) and 25 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal endothelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal endothelial cells in vitro.
ECM培养基是专门为正常人类微血管内皮细胞体外培养设计的最适于其生长的完全培养基。是经灭菌的液体培养基，包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和低浓度胎牛血清(5%)。该培养基缓冲体系为重碳酸盐，在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类微血管内皮细胞体外培养中的增殖和生长，并为其达到最理想营养平衡状态。
细胞培养基包含500 ml基础培养基，25mll胎牛血清（FBS, Cat. No. 0025） 、5ml 内皮细胞生长因子（ECGS, Cat. No. 1052 ）、5 ml青霉素/链霉素溶液(P/S, Cat. No. 0503) 。
1.) Li, F., Zhang, J., Luo, L., & Hu, J. (2023). Protective effects of xanthohumol against diabetic nephropathy in a mouse model. Kidney and Blood Pressure Research. https://doi.org/10.1159/000528650
2.) Lu, Y., Chen, X., Liu, X., Shi, Y., Wei, Z., Feng, L., Jiang, Q., Ye, W., Sasaki, T., Fukunaga, K., Ji, Y., Han, F., & Lu, Y.-M. (2023). Endothelial TFEB signaling-mediated autophagic disturbance initiates microglial activation and cognitive dysfunction. Autophagy, 1–18. https://doi.org/10.1080/15548627.2022.2162244
3.) Qu, R., Zhou, D., Guo, T., He, W., Cui, C., Zhou, Y., Zhang, Y., Tang, Z., Zhang, X., Wang, Q., Wang, T., & Zhang, Y. (2023). 4D printing of shape memory inferior vena cava filters based on copolymer of Poly(Glycerol Sebacate) acrylate-co-hydroxyethyl methacrylate (PGSA-Hema). Materials & Design, 225, 111556. https://doi.org/10.1016/j.matdes.2022.111556
4.) Wang, R., Xu, J., Tang, Y., Wang, Y., Zhao, J., Ding, L., Peng, Y., & Zhang, Z. (2023). Transcriptome-wide analysis reveals the coregulation of RNA-binding proteins and alternative splicing genes in the development of atherosclerosis. Scientific Reports, 13(1). https://doi.org/10.1038/s41598-022-26556-6
5.) Feng Y;Wang L;Dong C;Yang X;Wang J;Zhang X;Yuan Y;Dai J;Huang J;Yuan F; (2023, January). MicroRNA-376B-3P suppresses choroidal neovascularization by regulating glutaminolysis in endothelial cells. Investigative ophthalmology & visual science. Retrieved February 3, 2023, from https://pubmed.ncbi.nlm.nih.gov/36719700/
6.) Hu, Y., Liu, Z., Tao, X., Li, J., Hou, Z., Guo, X., Zhou, D., Wang, M., & Zhu, B. (2023). Epigallocatechin-3-gallate alleviates trans, trans-2,4-decadienal-induced endothelial pyroptosis and dysfunction by inhibiting NLRP3 inflammasome activation. Journal of Functional Foods, 101, 105428. https://doi.org/10.1016/j.jff.2023.105428
7.) Wang, J., Hua, M., Li, H., Xu, D., Li, F., & Xu, F. (2023). Circ_0007706 downregulation ameliorates neonatal hypoxic ischemic encephalopathy via regulating the Mir-579-3p/traf6 axis. Brain Research Bulletin, 194, 90–99. https://doi.org/10.1016/j.brainresbull.2023.01.013
8.) He, Q., Chen, Z., Li, J., Liu, J., Zuo, Z., Lin, B., Song, K., Zhou, C., Lai, H., Huang, Q., & Guo, X. (2023). SENP6-mediated desumoylation of VEGFR2 enhances its cell membrane transport in angiogenesis. International Journal of Molecular Sciences, 24(3), 2544. https://doi.org/10.3390/ijms24032544
9.) Liu, D., Song, Y., Song, T., Lin, L., Zhang, L., Yang, Q., Niu, X., Liang, D., Yin, J., Yang, W., & Zhang, D. (2023). RRP regulates autophagy through the AMPK pathway to alleviate the effect of cell senescence on atherosclerosis. Oxidative Medicine and Cellular Longevity, 2023, 1–22. https://doi.org/10.1155/2023/9645789
10.) Fang, J., Ji, Q., Gao, S., Xiao, Z., Liu, W., Hu, Y., Lv, Y., Chen, G., Mu, Y., Cai, H., Chen, J., & Liu, P. (2022). PDGF-BB is involved in HIF-1α/CXCR4/CXCR7 axis promoting capillarization of hepatic sinusoidal endothelial cells. Heliyon. https://doi.org/10.1016/j.heliyon.2022.e12715
11.) Lou, T., Chen, K., Luo, Q., Liu, C., Yuan, Y., & Fan, C. (2022). Periosteum-inspired in situ cap generated nanocomposite hydrogels with strong bone adhesion and superior stretchability for accelerated distraction osteogenesis. Biomaterials Research, 26(1). https://doi.org/10.1186/s40824-022-00330-1
12.) Seo, S., Jang, M., Kim, H., Sung, J. H., Choi, N., Lee, K., & Kim, H. N. (2022). Neuro‐glia‐vascular‐on‐a‐chip system to assess aggravated neurodegeneration via brain endothelial cells upon exposure to diesel exhaust particles. Advanced Functional Materials, 2210123. https://doi.org/10.1002/adfm.202210123
13.) Jia, Y., Wang, Q., Liang, M., & Huang, K. (2022). KPNA2 promotes angiogenesis by regulating STAT3 phosphorylation. Journal of Translational Medicine, 20(1). https://doi.org/10.1186/s12967-022-03841-6
14.) Abu, N., Akhtar, M.N., Yeap, S.K., Lim, K.L., Ho, W.Y., Abdullah, M.P., Ho, C.L., Omar, A.R., Ismail, J. & Alitheen, N.B. (2016) Flavokawain B induced cytotoxicity in two breast cancer cell lines, MCF-7 and MDA-MB231 and inhibited the metastatic potential of MDA-MB231 via the regulation of several tyrosine kinases In vitro BMC Complement Altern Med. 16
15.) Chen, H., Tang, N., Chen, M. & Chen, D. (2016) Endothelialization of TiO2 Nanorods Coated with Ultrathin Amorphous Carbon Films Nanoscale Research Letters. 11
16.) Drolez, A., Vandenhaute, E., Julien, S., Gosselet, F., Burchell, J., Cecchelli, R., Delannoy, P., Dehouck, M.P. & Mysiorek, C. (2016) Selection of a Relevant In Vitro Blood-Brain Barrier Model to Investigate Pro-Metastatic Features of Human Breast Cancer Cell Lines PLoS One. 11
17.) Fan, Y., Qiao, Y., Huang, J. & Tang, M. (2016) Protective Effects of Panax notoginseng Saponins against High Glucose-Induced Oxidative Injury in Rat Retinal Capillary Endothelial Cells Evid Based Complement Alternat Med. 2016
18.) He, Y., Luan, Z., Fu, X. & Xu, X. (2016) Overexpression of uncoupling protein 2 inhibits the high glucose-induced apoptosis of human umbilical vein endothelial cells Int J Mol Med. 37
19.) Hong, H., He, C., Zhu, S., Zhang, Y., Wang, X., She, F. & Chen, Y. (2016) CCR7 mediates the TNF-à-induced lymphatic metastasis of gallbladder cancer through the "ERK1/2 - AP-1" and "JNK - AP-1" pathways J Exp Clin Cancer Res. 35
20.) Hong, H., Jiang, L., Lin, Y., He, C., Zhu, G., Du, Q., Wang, X., She, F. & Chen, Y. (2016) TNF-alpha promotes lymphangiogenesis and lymphatic metastasis of gallbladder cancer through the ERK1/2/AP-1/VEGF-D pathway BMC Cancer. 16
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
Endothelial Cell Medium (ECM), when used with Endothelial Cell Growth Supplement (ECGS, Cat #1052) and 25 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal endothelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal endothelial cells in vitro.
ECM培养基是专门为正常人类微血管内皮细胞体外培养设计的最适于其生长的完全培养基。是经灭菌的液体培养基，包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和低浓度胎牛血清(5%)。该培养基缓冲体系为重碳酸盐，在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类微血管内皮细胞体外培养中的增殖和生长，并为其达到最理想营养平衡状态。
细胞培养基包含500 ml基础培养基，25mll胎牛血清（FBS, Cat. No. 0025） 、5ml 内皮细胞生长因子（ECGS, Cat. No. 1052 ）、5 ml青霉素/链霉素溶液(P/S, Cat. No. 0503) 。
1.) Li, F., Zhang, J., Luo, L., & Hu, J. (2023). Protective effects of xanthohumol against diabetic nephropathy in a mouse model. Kidney and Blood Pressure Research. https://doi.org/10.1159/000528650
2.) Lu, Y., Chen, X., Liu, X., Shi, Y., Wei, Z., Feng, L., Jiang, Q., Ye, W., Sasaki, T., Fukunaga, K., Ji, Y., Han, F., & Lu, Y.-M. (2023). Endothelial TFEB signaling-mediated autophagic disturbance initiates microglial activation and cognitive dysfunction. Autophagy, 1–18. https://doi.org/10.1080/15548627.2022.2162244
3.) Qu, R., Zhou, D., Guo, T., He, W., Cui, C., Zhou, Y., Zhang, Y., Tang, Z., Zhang, X., Wang, Q., Wang, T., & Zhang, Y. (2023). 4D printing of shape memory inferior vena cava filters based on copolymer of Poly(Glycerol Sebacate) acrylate-co-hydroxyethyl methacrylate (PGSA-Hema). Materials & Design, 225, 111556. https://doi.org/10.1016/j.matdes.2022.111556
4.) Wang, R., Xu, J., Tang, Y., Wang, Y., Zhao, J., Ding, L., Peng, Y., & Zhang, Z. (2023). Transcriptome-wide analysis reveals the coregulation of RNA-binding proteins and alternative splicing genes in the development of atherosclerosis. Scientific Reports, 13(1). https://doi.org/10.1038/s41598-022-26556-6
5.) Feng Y;Wang L;Dong C;Yang X;Wang J;Zhang X;Yuan Y;Dai J;Huang J;Yuan F; (2023, January). MicroRNA-376B-3P suppresses choroidal neovascularization by regulating glutaminolysis in endothelial cells. Investigative ophthalmology & visual science. Retrieved February 3, 2023, from https://pubmed.ncbi.nlm.nih.gov/36719700/
6.) Hu, Y., Liu, Z., Tao, X., Li, J., Hou, Z., Guo, X., Zhou, D., Wang, M., & Zhu, B. (2023). Epigallocatechin-3-gallate alleviates trans, trans-2,4-decadienal-induced endothelial pyroptosis and dysfunction by inhibiting NLRP3 inflammasome activation. Journal of Functional Foods, 101, 105428. https://doi.org/10.1016/j.jff.2023.105428
7.) Wang, J., Hua, M., Li, H., Xu, D., Li, F., & Xu, F. (2023). Circ_0007706 downregulation ameliorates neonatal hypoxic ischemic encephalopathy via regulating the Mir-579-3p/traf6 axis. Brain Research Bulletin, 194, 90–99. https://doi.org/10.1016/j.brainresbull.2023.01.013
8.) He, Q., Chen, Z., Li, J., Liu, J., Zuo, Z., Lin, B., Song, K., Zhou, C., Lai, H., Huang, Q., & Guo, X. (2023). SENP6-mediated desumoylation of VEGFR2 enhances its cell membrane transport in angiogenesis. International Journal of Molecular Sciences, 24(3), 2544. https://doi.org/10.3390/ijms24032544
9.) Liu, D., Song, Y., Song, T., Lin, L., Zhang, L., Yang, Q., Niu, X., Liang, D., Yin, J., Yang, W., & Zhang, D. (2023). RRP regulates autophagy through the AMPK pathway to alleviate the effect of cell senescence on atherosclerosis. Oxidative Medicine and Cellular Longevity, 2023, 1–22. https://doi.org/10.1155/2023/9645789
10.) Fang, J., Ji, Q., Gao, S., Xiao, Z., Liu, W., Hu, Y., Lv, Y., Chen, G., Mu, Y., Cai, H., Chen, J., & Liu, P. (2022). PDGF-BB is involved in HIF-1α/CXCR4/CXCR7 axis promoting capillarization of hepatic sinusoidal endothelial cells. Heliyon. https://doi.org/10.1016/j.heliyon.2022.e12715
11.) Lou, T., Chen, K., Luo, Q., Liu, C., Yuan, Y., & Fan, C. (2022). Periosteum-inspired in situ cap generated nanocomposite hydrogels with strong bone adhesion and superior stretchability for accelerated distraction osteogenesis. Biomaterials Research, 26(1). https://doi.org/10.1186/s40824-022-00330-1
12.) Seo, S., Jang, M., Kim, H., Sung, J. H., Choi, N., Lee, K., & Kim, H. N. (2022). Neuro‐glia‐vascular‐on‐a‐chip system to assess aggravated neurodegeneration via brain endothelial cells upon exposure to diesel exhaust particles. Advanced Functional Materials, 2210123. https://doi.org/10.1002/adfm.202210123
13.) Jia, Y., Wang, Q., Liang, M., & Huang, K. (2022). KPNA2 promotes angiogenesis by regulating STAT3 phosphorylation. Journal of Translational Medicine, 20(1). https://doi.org/10.1186/s12967-022-03841-6
14.) Abu, N., Akhtar, M.N., Yeap, S.K., Lim, K.L., Ho, W.Y., Abdullah, M.P., Ho, C.L., Omar, A.R., Ismail, J. & Alitheen, N.B. (2016) Flavokawain B induced cytotoxicity in two breast cancer cell lines, MCF-7 and MDA-MB231 and inhibited the metastatic potential of MDA-MB231 via the regulation of several tyrosine kinases In vitro BMC Complement Altern Med. 16
15.) Chen, H., Tang, N., Chen, M. & Chen, D. (2016) Endothelialization of TiO2 Nanorods Coated with Ultrathin Amorphous Carbon Films Nanoscale Research Letters. 11
16.) Drolez, A., Vandenhaute, E., Julien, S., Gosselet, F., Burchell, J., Cecchelli, R., Delannoy, P., Dehouck, M.P. & Mysiorek, C. (2016) Selection of a Relevant In Vitro Blood-Brain Barrier Model to Investigate Pro-Metastatic Features of Human Breast Cancer Cell Lines PLoS One. 11
17.) Fan, Y., Qiao, Y., Huang, J. & Tang, M. (2016) Protective Effects of Panax notoginseng Saponins against High Glucose-Induced Oxidative Injury in Rat Retinal Capillary Endothelial Cells Evid Based Complement Alternat Med. 2016
18.) He, Y., Luan, Z., Fu, X. & Xu, X. (2016) Overexpression of uncoupling protein 2 inhibits the high glucose-induced apoptosis of human umbilical vein endothelial cells Int J Mol Med. 37
19.) Hong, H., He, C., Zhu, S., Zhang, Y., Wang, X., She, F. & Chen, Y. (2016) CCR7 mediates the TNF-à-induced lymphatic metastasis of gallbladder cancer through the "ERK1/2 - AP-1" and "JNK - AP-1" pathways J Exp Clin Cancer Res. 35
20.) Hong, H., Jiang, L., Lin, Y., He, C., Zhu, G., Du, Q., Wang, X., She, F. & Chen, Y. (2016) TNF-alpha promotes lymphangiogenesis and lymphatic metastasis of gallbladder cancer through the ERK1/2/AP-1/VEGF-D pathway BMC Cancer. 16
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
