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Monoclonal antibody ER-MP20 is useful for the detection of macrophage precursor cells in mid-stage development (late CFU-M, monoblasts and monocytes). It is ideally suited for the detection of monocytes in bone marrow samples by FACS. ER-MP20 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 (anti F4/80, product T-2006) is recommended. ER-MP20 also detects a wide range of endothelial cells. The red R-Phycoerythrin label is particularly useful to avoid the greenish autofluorescence of some cells in their resting state.