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A3 Procainamide Glycan Synonyms: A3 N-linked oligosaccharide
LudgerTag procainamide labeled glycans can be analyzed by UHPLC, HPLC, ESI-MS, and LC-ESI-MS methods. Because of its improved ionization efficiency compared to 2-AB labeling it can permit identification of minor glycans (<1% relative peak area) by ESI-MS.
Description: Tri-sialylated, tri-antennary complex-type N-glycan (oligosaccharide). The product supplied is a mixture of isomers differing in the type of sialic acid linkages (Neu5Acα2-3 or Neu5Acα2-6) and the Gal residue linkage of one of the arms (Galβ1-4 or Galβ1-3).
Sources: A3 glycans are found on several mammalian glycoproteins including bovine serum fetuin (bSF). This product is typically purified from the oligosaccharide pool released from bSF by hydrazinolysis using a combination of HPLC and glycosidase digestion.
Form: Dry. Dried by centrifugal evaporation from an aqueous solution containing approximately 50 mmol dm-3 of ammonium acetate (added to minimize acid-catalysed desialylation).
Molecular Weight: 3098
Mass Free Dye: 235.33
Mass Added To Glycan: 219.172