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For release of O-linked glycans from glycoprotein therapeutics.
The Ludger Liberate Orela Kit has been developed for O-linked deglycosylation of glycoproteins. Released glycans have free reducing terminii to allow fluorescent tagging by reductive amination. The kit includes reagents and materials for O-linked deglycosylation of up to 12 glycoprotein samples analysed in parallel or two sets of 6 samples, typically up to 1 mg of glycoprotein per sample. This method of O-glycan release demonstrates significant reduction in peeling and reduces the number of sample handling steps prior to release.
