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经过测试的应用
| Positive WB detected in | COLO 320 cells, HepG2 cells, Caco-2 cells, HEK-293 cells, Jurkat cells |
| Positive IHC detected in |
human oesophagus cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| APPLICATION | DILUTION |
|---|---|
| Western Blot (WB) |
WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) |
IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |