DESCRIPTION The EZ DNA Methylation-Startup Kit provides the necessary tools for complete bisulfite conversion of DNA for methylation analysis. This kit includes reagents that allow for bisulfite conversion of purified DNA and DNA directly from blood, cells, and fresh or FFPE tissues without the prerequisite for upstream DNA purification. A fully methylated Universal Methylated Human DNA Standard is provided with a special primer set for PCR to assess conversion efficiency. Finally, a unique ZymoTaq DNA Polymerase is included for robust amplification of bisulfite-treated DNA. HIGHLIGHTS The complete solution for bisulfite conversion. This all-in-one kit contains reagents for bisulfite conversion of DNA without the need for purification, methylated human DNA with control primers, and a robust hot-start PCR polymerase that is specifically formulated for bisulfite-converted DNA. Designed for the first-time user requiring a consolidated product to control for bisulfite conversion. Kit includes: one EZ DNA Methylation-Direct kit, one fully methylated Universal Methylated Human DNA Standard, and ZymoTaq DNA Polymerase.