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mTeSR1 培养基
  • 商城价格 登录后可查看价格
  • 货号 85850
  • 品牌 Stemcell ( 经销商 )
  • CAS号
  • 规格/包装 500ml
  • 单位 套
  • 储存条件 2-8℃（有负20度组分）
  • 现货状态 一周
  • 数量

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Product Description
mTeSR™1 medium is a complete, serum-free, defined formulation designed for the feeder-free maintenance and expansion of human
embryonic stem (ES) cells
1,2 and human induced pluripotent stem (iPS) cells3-5 in the undifferentiated state. Complete mTeSR™1 medium
(Basal Medium + 5X Supplement) contains recombinant human basic fibroblast growth factor (rh bFGF) and recombinant human
transforming growth factor β (rh TGFβ). Addition of further growth factors is not required.
mTeSR™1 may be used with either Corning® Matrigel® hESC-Qualified Matrix (Corning Catalog #354277) or Vitronectin XF™
(Catalog #07180, a matrix developed and manufactured by Nucleus Biologics) as the culture matrix.
Each lot of mTeSR™1 5X Supplement is used to prepare complete mTeSR™1 medium and then performance-tested in a culture assay
using human pluripotent stem cells (hPSCs).
Product Information
CATALOG #
SIZE
COMPONENTS
85850
500 mL
• mTeSR™1 Basal Medium (400 mL)
• mTeSR™1 5X Supplement (100 mL)
85857
1 L
• mTeSR™1 Basal Medium (800 mL)
• mTeSR™1 5X Supplement (2 x 100 mL)
85870
10 x 500 mL
• mTeSR™1 Basal Medium (10 x 400 mL)
• mTeSR™1 5X Supplement (10 x 100 mL)
85875
25 x 500 mL
• mTeSR™1 Basal Medium (25 x 400 mL)
• mTeSR™1 5X Supplement (25 x 100 mL)
Component Storage and Stability
The following components are sold as part of the mTeSR™1 medium kits (see Product Information) and are not available for individual sale.
COMPONENT NAME
COMPONENT #
STORAGE
SHELF LIFE
mTeSR™1 Basal Medium (400 mL)
85851
Store at 2 - 8°C.
Stable until expiry date on label.
mTeSR™1 Basal Medium (800 mL)
85871
Store at 2 - 8°C.
Stable until expiry date on label.
mTeSR™1 5X Supplement
85852
Store at -20°C.
Stable until expiry date on label.
Preparation of Complete mTeSR™1 Medium
Use sterile technique to prepare complete mTeSR™1 medium (Basal Medium + 5X Supplement). The following example is for preparing
500 mL of complete medium. If preparing 1 L of complete medium, add 2 x 100 mL of 5X Supplement to 800 mL of Basal Medium. If
preparing other volumes, adjust accordingly.
NOTE: Thaw supplements or complete medium at room temperature (15 - 25°C) or overnight at 2 - 8°C. Do not thaw in a 37°C water bath.
1. Thaw mTeSR™1 5X Supplement and warm to room temperature. Mix thoroughly.
NOTE: mTeSR™1 5X Supplement may appear slightly cloudy after thawing. If this is noted, place in a 37°C water bath for
approximately 5 minutes, swirling occasionally until supplement becomes clear. Supplement must be free of cloudiness before adding
to basal medium (step 2).
NOTE: Once thawed, use supplement immediately or aliquot and store at ­20°C for up to 3 months. Do not exceed the shelf life of the
supplement. After thawing the aliquoted supplement, use immediately. Do not re-freeze.
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