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Product Advantages
- Supports high yield of MSCs from umbilical cord tissue
- Umbilical cord-derived MSCs consistently express MSC-specific markers and retain superior expansion and differentiation capabilities
- Complete animal component-free workflow compatible with MesenCult™-ACF portfolio
What's Included
- MesenCult™-ACF Plus Medium, 500 mL
- MesenCult™ Umbilical Cord Derivation 500X Supplement, 0.5 mL
- MesenCult™-ACF Plus 500X Supplement, 0.5 mL
- Animal Component-Free Cell Attachment Substrate, 3 x 1 mL
Overview
MesenCult™-ACF Plus Umbilical Cord Culture Kit includes standardized, animal component- and serum-free media for the derivation and expansion of human mesenchymal stromal cells (also known as mesenchymal stem cells; MSCs) from umbilical cord tissue. This kit
works together with complete MesenCult™-ACF Plus Medium for the long-term expansion of umbilical cord-derived MSCs.
MesenCult™-ACF Plus Umbilical Cord Medium must be used in conjunction with Animal Component-Free Cell Attachment Substrate and Animal Component-Free Cell Dissociation Kit (Catalog #05426) to provide a complete animal component-free (ACF) culture system. Components of Animal Component-Free Cell Attachment Substrate and Animal Component-Free Cell Dissociation Kit are pre-screened and tested for optimal cell adherence when cells are cultured with MesenCult™-ACF Plus Umbilical Cord Culture Kit and MesenCult™-ACF Plus Medium. For optimal animal component-free cryopreservation, MesenCult™-ACF Freezing Medium (Catalog #05490) is recommended for human MSCs previously cultured in MesenCult™ media.
NOTE: L-Glutamine (Catalog #07100) is required for preparation of MesenCult™-ACF Plus Umbilical Cord Medium and complete MesenCult™-ACF Plus Medium; see Preparation of Reagents and Materials.
CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate.
works together with complete MesenCult™-ACF Plus Medium for the long-term expansion of umbilical cord-derived MSCs.
MesenCult™-ACF Plus Umbilical Cord Medium must be used in conjunction with Animal Component-Free Cell Attachment Substrate and Animal Component-Free Cell Dissociation Kit (Catalog #05426) to provide a complete animal component-free (ACF) culture system. Components of Animal Component-Free Cell Attachment Substrate and Animal Component-Free Cell Dissociation Kit are pre-screened and tested for optimal cell adherence when cells are cultured with MesenCult™-ACF Plus Umbilical Cord Culture Kit and MesenCult™-ACF Plus Medium. For optimal animal component-free cryopreservation, MesenCult™-ACF Freezing Medium (Catalog #05490) is recommended for human MSCs previously cultured in MesenCult™ media.
NOTE: L-Glutamine (Catalog #07100) is required for preparation of MesenCult™-ACF Plus Umbilical Cord Medium and complete MesenCult™-ACF Plus Medium; see Preparation of Reagents and Materials.
CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate.
Specialized Media
Mesenchymal Stromal Cells
Human
Cell Culture, Cell Isolation
MesenCult
Cell Line Development, Stem Cell Biology
Animal Component-Free