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Overview
Trypsin-EDTA (0.05%) can be used to achieve a single-cell suspension of human embryonic stem (ES) cells or human induced pluripotent stem (iPS) cells for flow cytometry or cloning assays. This is a gentler version than the standard concentration (0.25%), and has applications to many other cell culture systems in addition to human ES cells and iPS cells.
SUBTYPE
SPECIES
CONTAINS
• 0.5 g/L Porcine trypsin
• Hanks' Balanced Salt Solution (without Ca++ and Mg++)
• 0.2 g/L EDTA•4Na
• Hanks' Balanced Salt Solution (without Ca++ and Mg++)
• 0.2 g/L EDTA•4Na
Enzymatic
Human, Mouse, Rat, Non-Human Primate, Other