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Overview
MethoCult™ SF M3436 is optimized for the growth and enumeration of primitive erythroid progenitor cells (BFU-E) in colony-forming unit (CFU) assays of mouse bone marrow, fetal liver, spleen, peripheral blood, and purified cells. MethoCult™ SF M3436 does not support the growth of granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M) or multipotential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM). This formulation is serum-free and compatible with STEMvision™ software for automated colony counting of mouse bone marrow CFU assays. MethoCult™ SF M3436 can also be used for growth and enumeration of BFU-E in CFU assays of rat bone marrow cells.
Browse our Frequently Asked Questions (FAQs) on performing the CFU assay.
SUBTYPE
CELL TYPE
SPECIES
APPLICATION
BRAND
AREA OF INTEREST
FORMULATION
Browse our Frequently Asked Questions (FAQs) on performing the CFU assay.
CONTAINS
• Methylcellulose in Iscove's MDM
• Bovine serum albumin
• Recombinant human insulin
• Human transferrin (iron-saturated)
• 2-Mercaptoethanol
• Cytokines (including recombinant human erythropoietin [EPO])
• Supplements
• Bovine serum albumin
• Recombinant human insulin
• Human transferrin (iron-saturated)
• 2-Mercaptoethanol
• Cytokines (including recombinant human erythropoietin [EPO])
• Supplements
Semi-Solid Media, Specialized Media
Hematopoietic Stem and Progenitor Cells
Mouse, Rat
Cell Culture, Colony Assay, Functional Assay
MethoCult
Drug Discovery and Toxicity Testing, Stem Cell Biology
Serum-Free