The pJTI™ R4 Exp CMV EmGFP pA vector is a positive control vector for assessing the success of a retargeting reaction with a Jump-In™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector) included in the Jump-In™ parental kits, the EmGFP will be expressed and positive cells will fluoresce green. Ensure the Success of Your Jump-In™ Retargeting Reactions Successful retargeting of Jump-In™ parental cell lines like the the Jump-In™ GripTite™ HEK293 kit (A14150) is dependent on a variety of factors such as: Transfection efficiency • Cell confluency • Antibiotic selection conditions • Quality and concentration of DNA • Retargeting vector to integrase vector ratio We strongly recommend including a retargeting reaction with the pJTI™ R4 Exp CMV EmGFP pA vector in your Jump-In™ experiment along with negative controls (no plasmid DNA, no integrase vector) so you can easily visualize the results and optimize the retargeting conditions. For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. 规格 适用于（应用） Stable Cell Line Development, Targeted Integration 产品线 Jump-In™ 构成或诱导系统 组成型 输送类型 转染 产品规格 Liquid 促进剂 CMV 产品类型 Mammalian Expression Vector 选择试剂（真核生物） 无 运输条件 干冰 蛋白标记 未标记 克隆方法 Gateway™ 载体 Jump-In 载体 内容与储存 接收后立即将载体储存于 -20°C 下