The Applied Biosystems TaqMan Advanced miRNA Human A and B 96-Well Plates, Standard, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well standard plate format. This product contains a set of 8 plates for profiling of up to 752 unique miRNAs, and includes endogenous and exogenous miRNA controls for normalization of data results. Features of the TaqMan Advanced miRNA Human A and B 96-Well Plates include: • Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability • Sample savings—requires only 4 μL of purified plasma/serum or 2–20 ng of total RNA per sample • Updated content—assay content updated for mirBase v.21 • Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required • Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families Product details: Number of plates: 8 Number of unique miRNAs (not including exogenous controls): 752 Exogenous controls: cel-miR-39-3p, ath-miR159a Endogenous control: hsa-miR-16-5p Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex Recommended master mix: TaqMan Fast Advanced Master Mix Notes • TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples. • These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.