The resDNASEQ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kit with PrepSEQ Residual DNA Sample Preparation Kit is a quantitative PCR (qPCR)-based system for the detection of residual kanamycin-resistant plasmid used in the development of gene therapies, cell-based vaccines, and similar biotherapeutics. Reliable and rapid, the resDNASEQ system enables sensitive and specific quantitation of residual kanamycin-resistant plasmid DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples with different sample matrices to bulk drug substance—whether the sample contains high molecular weight or sheared DNA. This product includes sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts. For the quantitation kit alone, please see Cat. No. A50337. • Designed to quantitate residual plasmid DNA with any common alleles of kanamycin resistance gene • Highly sensitive quantitation through innovative DNA-free master mix • Highly sensitive quantitation using proven TaqMan real-time PCR technology • Includes master mix, TaqMan primer/probe mix, kanamycin-resistant plasmid DNA standard, and sample preparation reagents Integrated workflow solution The resDNASEQ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kit with PrepSEQ Residual DNA Sample Preparation Kit is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing. The included PrepSEQ Residual DNA Sample Preparation Kit offers both a manual and automated sample preparation workflow. The resDNASEQ kit has been validated on the Applied Biosystems 7500 Fast Real-Time PCR System and the QuantStudio 5 Real-Time PCR System. Data analysis is streamlined using AccuSEQ Real-Time PCR Detection Software that provides accurate quantitation and security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance. Easy to use With the resDNASEQ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kit, you perform qPCR analysis to compare the amount of kanamycin-resitant plasmid DNA in your test samples to standard curves generated with known amounts of kanamycin-resistant plasmid DNA standard. The resDNASEQ kit includes the components you need to carry out these steps, from master mix to TaqMan primer/probe mix to DNA standard, as well as step-by-step instructions.