1. 产品名称：气道上皮培养基

2.英文名称：CnT-Prime Airway Diff, Epithelial Culture Medium

3.产地/品牌：瑞士 Cellntec

4.国产/进口：进口

5.型号：500 mL (frozen)

6.货号：CnT-PR-AD

7.详细描述：

CnT-PR-AD

CnT-Prime气道Diff是一种完全定义的低钙(0.07 mM)培养基，旨在优化初级气道上皮细胞的分化。它完全没有动物或人为成分。CnT-Prime Airway Diff含有一系列氨基酸、矿物质、维生素和有机化合物，以及可以促进分化的改良生长因子。它不含苯酚红或抗生素/抗真菌药。

物种

主要用于人类气道上皮细胞。也可用于其他物种，包括老鼠。

组织类型

上皮细胞

规格

500毫升瓶，充分补充生长因子。不需要进一步增加。

产品使用

用于在完全确定的环境中促进原发性气道上皮细胞的终末分化。为了获得最佳的分化，上皮细胞必须生长到汇合，然后再加钙。有关详细说明，请参阅www.cellntec.com参考资料部分中的差异化协议。可用于2D和3D/气举培养中细胞的分化。

媒质类型

3 d-diff

定义

是的

ACF

是的

培养条件

这种介质是为在5%的二氧化碳大气中使用而设计的。有关建议的隔离、传递、区分和冻结协议，请访问www.cellntec.com的参考资料部分。

注意

对于常规细胞培养，CELLnTEC不建议使用抗生素/抗真菌药。这在分化过程中尤为重要。

储存/保质期

冷藏至零下15度。最好在日期之前，看标签。为了准备使用的培养基，最好在4摄氏度时解冻。另外，培养基可以在室温下解冻5-6小时。如在室温下解冻，在解冻过程结束时，确保不超过15℃。不要在热水中解冻。一旦解冻，培养基在黑暗中4℃的温度下可以保存6周。某些培养媒介成分对光线非常敏感。在任何时候都尽量减少暴露在阳光下。

质量控制

通过渗透压、pH值和各种离子浓度测试介质的组成。媒质功能通过评估至少2个通道的生长和形态或原发性人体上皮细胞来测试。无细菌，真菌和支原体污染。

运输条件

冷冻运输。

预期用途

只供研究用途。不用于治疗或诊断。

CnT-Prime Airway Diff, Epithelial Culture Medium

CnT-Prime Airway Diff is a fully defined, low calcium (0.07 mM) medium formulation designed for optimal differentiation of primary airway epithelial cells. It is completely free of animal or human-derived components. CnT-Prime Airway Diff contains a full range of amino acids, minerals, vitamins and organic compounds, and modified growth factors to encourage differentiation. It does not contain phenol-red, or antibiotics / antimycotics.

SPECIES

Developed for primary human airway epithelial cells. May be used for other species as well, including mouse.

TISSUE TYPE

Epithelia

PACK SIZE

500 mL bottle, fully supplemented with growth factors. No further additions required.

PRODUCT USE

Developed to encourage terminal differentiation of primary airway epithelial cells in a fully defined environment. For optimal differentiation, epithelial cells must be grown to confluence, and then additional calcium added. For detailed instructions, please see the differentiation protocols in the resources section of www.cellntec.com. May be used to differentiate cells in both 2D and 3D/airlift cultures.

MEDIA TYPE

3D-Diff

DEFINED

Yes

ACF

Yes

CULTURE CONDITION

This medium is designed for use in a 5% CO2 atmosphere. For suggested isolation, passaging, differentiation and freezing protocols, please visit the resources section of www.cellntec.com

NOTE

For routine cell cultivation, CELLnTEC does not recommend the use of antibiotics / antimycotics. This is particularly important during differentiation.

STORAGE / SHELF LIFE

Store frozen below -15oC. For best before date, see label. To prepare medium for use, it is preferable to thaw overnight at 4oC. Alternatively, medium may be thawed at room temperature for approx 5-6 hours. If thawing at room temperature, ensure contents do not rise above 15oC at the end of the thawing process. Do not thaw in a hot water bath. Once thawed, medium has a remaining shelf-life of 6-weeks when stored at 4oC in the dark. Certain culture media components are very sensitive to light. Minimize light exposure at all times.

QUALITY CONTROL

Media composition is tested via osmolality, pH and the concentration of various ions. Media functionality is tested by evaluating growth and morphology or primary human epithelial cells over at least 2 passages. Free of bacteria, fungi and mycoplasma contamination.

SHIPPING CONDITION

Medium is shipped frozen.

INTENDED USE

For research use only. Not for use in therapy or diagnostics.

LAST UPDATE

2017-09-27