描述

特异性:

Anti-Mouse IFNAR1(克隆MAR1-5A3)识别细胞外的域IFNAR1的老鼠受体亚基IFN-α/β

抗原分布:

IFNAR1和IFNAR2在几乎所有细胞上都有共表达。

该抗体是专门为体内功能检测而制备的，它可以阻断I型IFN受体在体内和体外的信号传导，而不会耗尽IFNAR1承载细胞。该抗体由IFNAR1基因敲除小鼠体内基因免疫产生，其质粒编码鼠IFNAR1细胞外区域。IFNAR1和IFNAR2 coexpressed几乎所有细胞和弥补heterodimeric受体结合所有的I型干扰素(干扰素α和β)。I型IFNs是一种细胞因子家族，已被证明能够促进体内的抗病毒、抗微生物、抗肿瘤和自身免疫反应。

已知物种交叉反应:Ms+， Hu-

主要宿种

小鼠

免疫原

该抗体由IFNAR1基因敲除小鼠体内基因免疫产生，其质粒编码鼠IFNAR1细胞外区域。

配方

该抗体无菌包装，在0.01 M磷酸盐缓冲盐水(PBS) pH 7.2、150mm NaCl中配制，不添加任何载体蛋白、钾或防腐剂。

纯度

采用SDS-PAGE和高效液相色谱(HPLC)联用法测定了血蓝比(>)98%

内毒素

LAL法测定<0.5 EU/mg

存储和稳定

这个抗体是稳定的,至少一个星期无菌存储时2 - 8°C。长期存储无菌整除工作卷没有稀释并存储在手动除霜冰箱-20°C。避免反复的冻融循环。

报告应用程序

FC, N, IP, WB, B

阻塞:克隆MAR1-5A3的半衰期很短，基本上是因为每个细胞都表达IFNAR1受体，受体循环非常迅速。而且，如果你想在体内阻断功能，你需要确保所有的受体在所有的区域都被持续阻断。因此，你需要一个大的体内负载剂量(2.5 mg/小鼠)来饱和体内所有的结合位点，然后保持足够高的水平来保持它们的饱和。对于体内阻断研究，我们建议给负荷剂量为2.5毫克/只老鼠，然后每周给0.5毫克/只老鼠。2。5毫克负载剂量后的半衰期约为5天。[然而，如果你只注射250微克的低剂量，那么它的半衰期是1.5天——因为你没有给老鼠注射饱和剂]。

每个研究者都应该为特定的应用确定他们自己的最佳工作稀释度。

Description

Specificity:

Mouse Anti-Mouse IFNAR1 (Clone MAR1-5A3) recognizes the extracellular domain of the IFNAR1 subunit of the mouse IFN-α/β receptor.

Antigen Distribution:

IFNAR1 and IFNAR2 are coexpressed on nearly all cells.

Background:

The antibody when prepared specifically for In vivo functional assays blocks type I IFN receptor signaling both In vitro and In vivo without depleting IFNAR1 bearing cells. This antibody was produced by In vivo genetic immunization of IFNAR1 knockout mice with a plasmid encoding the extracellular domain of murine IFNAR1. IFNAR1 and IFNAR2 are coexpressed on nearly all cells and make up the heterodimeric receptor that binds all type I IFNs (IFN α and β). Type I IFNs are a family of cytokines that have been shown to promote anti-viral, anti-microbial, anti-tumor and autoimmune responses In vivo.

Known Species Cross Reactivity: Ms+, Hu-

Host Species

Mouse

Immunogen

This antibody was produced by In vivo genetic immunization of IFNAR1 knockout mice with a plasmid encoding the extracellular domain of murine IFNAR1.

Formulation

This antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Purity

>98% by SDS-PAGE and HPLC

Endotoxin

<0.5 EU/mg as determined by the LAL method

Storage and Stability

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Reported Applications

FC, N, IP, WB, B

Blocking: Clone MAR1-5A3 has a short half-life, basically because every cell expresses the IFNAR1 receptor and the receptor recycles very rapidly. And, if you want to block function in vivo, you need to be sure that all of the receptors are blocked continually in all compartments. Therefore, you need a large in vivo loading dose (2.5 mg/mouse) to saturate all the binding sites in vivo and then maintain a high enough level to keep them saturated. For in vivo blocking studies we recommend give a loading dose of 2.5 mg/mouse and follow with a weekly dose of 0.5 mg/mouse. The half-live following a 2.5 mg loading dose is about 5 days. [However, if you only inject a low dose of 250 micrograms, then the half life is 1.5 days – because you haven’t saturated the mouse].

Each investigator should determine their own optimal working dilution for specific applications.