产品名：重组人长链R3胰岛素样生长因子（LR3-IGF 1）

产品号：LT0107

产品信息：LR3-IGF I 是应用于细胞培养的无动物成分的关键细胞因子，是专门为无血清或低血清细胞培养打造的，用于细胞增殖、延长寿命，并通过抗凋亡及促进细胞增殖进一步提高蛋白表达，是用于替换insulin的理想选择。

别名：Long R3 IGF-I, Insulin-like Growth Factor-I, Somatamedin C, IGF-IA

英文描述：

The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds. IGF-I LR3 is a recombinant analog of human IGF-I comprised of the complete IGF-I sequence, with an Arginine substitution for the third position Glutamic acid, and a 13 amino acid length N terminus peptide extension. Specifically engineered for higher biological potency in vitro, IGF-I LR3 has an increased half-life and a binding aversion to native proteins within the body that make it ideal for both research and large-scale culturing. Recombinant Human IGF-I LR3 is a 9.1 kDa, single, non-glycosylated polypeptide chain containing 83 amino acid residues.

氨基酸序列:

MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

来源:

E.coli

保存条件：

4℃保存6个月，-20℃保存一年或者-80℃长期保存

纯度&内毒素：

Greater than 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin Level<0.5EU/μg

The purity of rh LR3-IGF 1 was determined by SDS-PAGE of reduced rh LR3-IGF I and staining overnight with Coomassie Blue.