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2023特价,Agilent 安捷伦(Stratagene)210518 QuikChange Lightning Site-Directed Mutagenesis Kit 定点突变试剂盒
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  • 货号 210518
  • 品牌 Agilent/安捷伦 ( 经销商 )
  • CAS号 承诺原装正品
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QuikChange Lightning 10 rxn,美国Stratagene公司是世界生命科学研究领域致力于发展新产品和新技术的领导者。经典产品包括:文库构建试剂盒,预制文库,Quik-Change定点突变,双杂交系统,腺病毒表达系统,高灵敏度特异性QPCR试剂,超高保真Pfu酶,基因/蛋白功能分析,支原体检测等。


Agilent 安捷伦(Stratagene)210518 QuikChange Lightning Site-Directed Mutagenesis Kit 定点突变试剂盒

主要用于点突变,插入突变和缺失突变,采用新的突变酶系统,可扩增14 kb大小的模板,仅需3个小时即可完成。突变用酶是QuikChange Lightning Enzyme(包括PfuUltra High-Fidelity DNA 聚合酶在内的混合酶和经优化的Dpn I)。

Agilent 安捷伦(Stratagene)210518/210519 QuikChange Lightning Site-Directed Mutagenesis Kit 定点突变试剂盒
QuikChange Lightning - Details & Specifications

In vitro site-directed mutagenesis is an invaluable technique for characterizing the dynamic, complex relationships between protein structure and function, for studying gene expression elements, and for carrying out vector modification. Several approaches to this technique have been published, but these methods generally require single-stranded DNA (ssDNA) as the template and are labor intensive or technically difficult. Our QuikChange Lightning Site-Directed Mutagenesis Kit allows site specific mutation in virtually any double-stranded plasmid, thus eliminating the need for subcloning and for ssDNA rescue. In addition, the QuikChange Lightning Site-Directed Mutagenesis Kits do not require specialized vectors, unique restriction sites, multiple transformations or in vitro methylation treatment steps. The simple, rapid three-step generates mutants with high efficiency.

QuikChange Lightning Site-Directed Mutagenesis Kit

Using the most advanced high fidelity enzyme technology, the protocols have been accelerated while maintaining the highest accuracy for site-directed mutagenesis (see Figure 1). Exclusive to the QuikChange Lightning Site-Directed Mutagenesis Kit is a proprietary Pfu-based polymerase blend and the newly optimized Dpn I enzyme, which together allow for mutagenesis in approximately one hour, plus an overnight transformation.

 

Figure 1. Overview of the QuikChange Lightning Site-directed Mutagenesis Method.


The QuikChange Lightning Site-Directed Mutagenesis Kit delivers mutant plasmids up to three times faster than our original QuikChange kits, without losses in mutagenesis efficiency or accuracy. The kit has been optimized for mutagenesis of plasmids of up to 14 kb, allowing rapid, efficient, and accurate mutagenesis of small and large plasmids with a single kit.

QuikChange Lightning Multi Site-Directed Mutagenesis Kit

The QuikChange Lightning Multi Site-Directed Mutagenesis Kit offers the most rapid and reliable method for site-directed mutagenesis of plasmid DNA at up to five different sites simultaneously. Using the most advanced high fidelity enzyme technology, the protocols have been accelerated while maintaining the highest accuracy for site-directed mutagenesis. No specialized vectors or unique restriction sites are needed–virtually any plasmid of up to 8 kb is a suitable template. The rapid three-step procedure used for the QuikChange Lightning Multi kit introduces mutations at three different sites simultaneously in the 4-kb control plasmid with greater than 55% efficiency.

As with our original QuikChange Multi kit, a single mutagenic oligonucleotide is required to mutagenize each site, using a double-stranded DNA template. The kit’s exclusive enzyme blend features a unique Pfu Fusion-based DNA polymerase, providing high fidelity DNA synthesis. The rapid PCR cycling parameters of the unique enzyme blend, together with our newly optimized Dpn I enzyme, allow for multi-site mutagenesis in approximately two hours, plus an overnight transformation. The QuikChange Lightning Multi Site-Directed Mutagenesis Kit delivers results up to three times faster than our original multi-site mutagenesis kit (see Figure 2).

Figure 2. QuikChange Lightning Multi Saves Hours of Valuable Research Time.

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