Mitochondrial membrane potential assay kit that uses JC-10. Compatible with fluorescence microscopy, flow cytometry, and plate reader assays. Healthy: Ex 540 nm / Em 590 nm | Compromised: Ex 490 nm / Em 525 nm ION Vital - MitoVolt assay kit is the ideal solution for detecting changes in mitochondrial membrane potential due to cell apoptosis or other stress-inducing phenomena. MitoVolt can be used to monitor and/or visualize mitochondrial membrane potential as a static endpoint or in real-time. When following our protocol, the MitoVolt kit provides enough reagents to make 25 mL of working solution, enough for five 96-well plates or 500 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations and assay volumes for your application. JC-10 is a mitochondrial membrane potential probe. It possesses superior aqueous solubility compared to its better known analogue, JC-1. At low concentrations, JC-10 is monomeric and emits a green fluorescence. JC-10 accumulates in healthy mitochondria, forming J-aggregates that exhibit an orange fluorescence. Mitochondrial depolarization, a key marker of cellular apoptosis, results in a loss of JC-10 accumulation and a reversal to its monomeric form. This reversible behavior of JC-10 allows for ratiometric analysis of mitochondrial membrane potential, where a shift from orange (Ex/Em: 540nm/590nm) to green fluorescence (Ex/Em: 490nm/525nm) is indicative of compromised mitochondria.