HMGB1 Isoforms Kit is an explorative kit thought to test different redox isoforms of HMGB1. The kit contains 3 different isoforms of HMGB1: 10 ug of Fully reduced HMGB1 10 ug of Disulfide HMGB1 10 ug of Terminally oxidized HMGB1 Different activities of HMGB1 have been attributed to different biochemical forms that bind to specific receptors (Yang et al.,2012). Fully reduced HMGB1 has all the three cysteines completely reduced and is a potent chemoattractanct for many cell types by forming a heterocomplex with the chemokine CXCL12 and signaling via CXCR4 (rif PMCID: PMC3302219 ). Disulfide HMGB1 contains a disulfide bond between cysteines C23 and C45 and acts as a proinflammatory mediator by binding to TLR4 and RAGE receptors (Yang et al, 2012; Tirone et al, 2018). Terminally oxidized HMGB1 has all the cysteines oxidized to sulfonates and has no activity, either as a chemoattractant or in cytokine stimulation (Kazama et al,2008;Yang et al, 2012).It can be useful as negative control in your experiment. The proteins we provide are the natural proteins, with no tags or additional amino acids.